NEW PYRUVATE-KINASE VARIANT (PK OSAKA) DEMONSTRATED BY PARTIAL-PURIFICATION AND CONDENSATION
- 1 January 1976
- journal article
- research article
- Vol. 28 (5), 474-481
Abstract
A qualitative variant of erythrocyte (R) and liver (L) pyruvate kinases (PK Osaka) was detected in a family in which 2 siblings have extremely low PK activity by semipurification with DEAE cellulose chromatography and subsequent concentration of the enzyme solutions. This was reported to be a quantitative variant based on studies of crude tissue preparations. The molecular aberrations were characterized by slow mobility upon electrophoresis, abnormal kinetics for phosphoenolpyruvate and low affinity for anti-human erythrocyte PK serum. The mutant PK L was similiar both electrophoretically and immunologically to PK R.This publication has 21 references indexed in Scilit:
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