Development of a quantitative gene expression assay for Chlamydia trachomatis identified temporal expression of σ factors
- 21 September 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 458 (3), 354-358
- https://doi.org/10.1016/s0014-5793(99)01182-5
Abstract
Chlamydia trachomatis is an important human pathogen which possesses a unique bi-phasic developmental cycle. We used lightcycler methodology to quantitatively measure gene transcript levels in C. trachomatis strain L2. By measuring 16S rRNA transcript levels, we determined C. trachomatis L2 to have a generation time of approximately 3 h and an inclusion burst size of 200–300 particles. The three chlamydial σ factor genes rpoD (σ66), rpsD (σ28) and rpoN (σ54) exhibited different patterns of temporal expression. rpoD was central to early chlamydial development, whereas rpsD and rpoN were temporally expressed, coinciding with elementary body (EB) to reticulate body (RB) conversion and RB to EB conversion, respectively.Keywords
This publication has 26 references indexed in Scilit:
- Genome Sequence of an Obligate Intracellular Pathogen of Humans: Chlamydia trachomatisScience, 1998
- Eubacterial sigma-factorsFEMS Microbiology Reviews, 1998
- Developments in Quantitative PCRcclm, 1998
- The immunobiology and immunopathology of chlamydial infectionsAPMIS, 1995
- Chlamydiae and the biochemistry of intracellular parasitismTrends in Microbiology, 1994
- A developmentally regulated chlamydial gene with apparent homology to eukaryotic histone H1.Proceedings of the National Academy of Sciences, 1992
- A chlamydial plasmid is differentially transcribed during the life cycle of Chlamydia trachomatisPlasmid, 1991
- Chlamydia trachomatis contains a protein similar to the Legionella pneumophila mip gene productMolecular Microbiology, 1991
- Sulfur-rich proteins of Chlamydia trachomatis: developmentally regulated transcription of polycistromic mRNA from tandem promotersGene, 1990
- Chlamydial InfectionsAnnual Review of Medicine, 1988