Quantitation of conductance pathways in antral gastric mucosa.
Open Access
- 1 May 1975
- journal article
- Published by Rockefeller University Press in The Journal of general physiology
- Vol. 65 (5), 645-662
- https://doi.org/10.1085/jgp.65.5.645
Abstract
The magnitude of cellular and shunt conductance of Necturus gastric antral mucosa was studied by (a) comparing the cellular PD response to transepithelial PD response during changes of ionic activity in the serosal bathing solution and (b) by measurement of current spread within the epithelial sheet. Using constant product KCl changes cellular resistance was 6,788 omegacm2 and shunt resistance was 1,803 omegacm2. Deletion of HCO3- from the serosal solution produced similar but quantitatively smaller changes in PD. Using HCO3- deletion cellular resistance was 7,338 omegacm2 and shunt resistance was 1,973 omegacm2. Measurement of current spead within the mucosa avoids changing ionic gradients yet gave very similar results; cellular resistance was 8,967 omegacm2 and shunt resistance was 2,947 omegacm2. The shunt contribution to transepithelial conductance ranged from 75.2 to 79.0%. Shunt selectivity was assessed using KCl dilution potentials, where mucosal dilution gave a small change in tissue PD compatible with an anion/cation selectivity ratio of 1.16 across the shunt, whereas serosal dilution effect was dominated by a PD change across the serosal membrane of the cell.Keywords
This publication has 9 references indexed in Scilit:
- Microelectrode studies of fundic gastric mucosa: Cellular coupling and shunt conductanceThe Journal of Membrane Biology, 1974
- Passive Electrical Properties of Toad Urinary Bladder EpitheliumThe Journal of general physiology, 1974
- Response of the Frog Skin to Steady-State Voltage ClampingThe Journal of general physiology, 1972
- Molecular factors in antral permeation: their proposed role in gastrin releaseAmerican Journal of Physiology-Legacy Content, 1971
- Studies on the Electrical Potential Profile across Rabbit IleumThe Journal of general physiology, 1971
- A COMPARISON OF THE ELECTRICAL RESISTANCES OF THE SURFACE CELL MEMBRANE AND CELLULAR WALL IN THE PROXIMAL TUBULE OF THE NEWT KIDNEYThe Japanese Journal of Physiology, 1967
- The Transport of Salt and Water across Isolated Rat IleumThe Journal of general physiology, 1967
- Profile of pH, Pressure, and Potential Difference at Gastroduodenal Junction in ManGastroenterology, 1965
- Potential difference across the pyloric antrumAmerican Journal of Physiology-Legacy Content, 1959