Bridging Reagent for Protein

Abstract

1.N⁶, N⁶-Hexamethylenebiscarbamoyllysine was synthesized by treating the copper complex of lysine with hexamethylenediisocyanate. The properties of this compound were described. 2. Ribonuclease [EC 2.7.7.16] was treated with hexamethylenediisocyanate. The derivative, in which 1.8 moles of the lysine residues reacted with hexamethylenediisocyanate, had the full activity. The fully active derivative had similar intrinsic viscosity and sedimentation coefficient to that of ribonuclease. It seemed therefore to have one intra-molecular bridge of hexamethylenediisocyanate. 3.Chymotrypsin [EG 3.4.4.5] treated with hexamethylenediisocyanate was obtained as an insoluble and enzymatically active derivative. The activity of the insoluble fraction was about 30%of the original.