High-frequency Protoplast-transfection ofStreptomyces parvulus2297 with Actinophage R4 DNA
- 1 October 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 44 (10), 2425-2428
- https://doi.org/10.1080/00021369.1980.10864344
Abstract
High frequency transfection of Streptomyces parvulus with actinophage R4 DNA was performed by modifying the procedure of protoplast transformation of S. coelicolor A3(2) with SCP2 plasmid DNA [Bibb et al., Nature, 274, 398 (1978)]. Optimum conditions for protoplast transfection included the presence of 16~24% (w/v) polyethyleneglycol 4000, and the maximum efficiency of transfection was 3 × 10−5 per phage DNA molecule. This value was at least 100 times higher than the efficiency of previously reported transfection systems in Streptomyces.This publication has 4 references indexed in Scilit:
- A New, Wide Host-range, Temperate Bacteriophage (R4) of Streptomyces and its Interaction with some Restriction-Modification SystemsJournal of General Microbiology, 1979
- High-frequency fusion of Streptomyces parvulus or Streptomyces antibioticus protoplasts induced by polyethylene glycolJournal of Bacteriology, 1979
- Genetic Determination of Methylenomycin Synthesis by the SCP1 Plasmid of Streptomyces coelicolor A3(2)Microbiology, 1977
- Factors Affecting Infection of Protoplasts with Deoxyribonucleic Acid of Actinophage PK-66Journal of Virology, 1968