SIMPLE TECHNIQUE FOR MASS CULTIVATION OF CAMPYLOBACTER FETUS

Abstract

Studies using 86 media for maximum growth of C. fetus for antigen production showed that a diphasic medium (solid base with liquid overlay) was most suitable. The solid base was double strength cystine heart agar. The liquid overlay was thioglycollate medium of Brewer (135-C) without agar. This medium yielded maximum growth of C. fetus in 6 days with good motility, less clumping and less filament formation than all other media tried. [Large quantities of C. fetus are required for agglutination tests in diagnosis in animals and for vaccine preparation.].