SIMPLE AND RAPID STAINING FOR DETECTION OF ENTAMOEBA CYSTS AND OTHER PROTOZOANS WITH FLUOROCHROMES
- 1 January 1987
- journal article
- research article
- Published by Editorial Committee of Japanese Journal of Infectious Diseases, National Institute of Infectious Dis in Japanese Journal of Medical Science and Biology
- Vol. 40 (1), 35-46
- https://doi.org/10.7883/yoken1952.40.35
Abstract
Three fluorochromes were applied to stain various parasitic protozoans. By double staining with 4'',6-diamidino-2-phenylindole and propidium iodide, differentiation of the nuclei from the cytoplasm can easily be achieved within several seconds. The chromatoid bodies in Entamoeba cysts were stained bright red. Plasmodium yoelii at all stages except late trophozoites and young gametocytes was easily identified. In the oocysts of Cryptosporidium sp., the nuclei and cytoplasm of the sporozoites fluoresced bluish white and red, respectively, whereas the residual body appeared blue or green. The third fluorochrome , Calcofluor white M2R, was suitable for detecting the cysts of Entamoeba spp. and Chilomastix mesnili.This publication has 13 references indexed in Scilit:
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