The relation between heat activation and colony formation for the spores of Bacillus stearothermophilus
- 1 November 1964
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Pharmacy and Pharmacology
- Vol. 16 (11), 725-732
- https://doi.org/10.1111/j.2042-7158.1964.tb07396.x
Abstract
Spores suspended in water were heated at 73·5, 100, 115 and 121° and colony counts made. These showed an initial increase followed by an exponential decline. Spores in phosphate buffer showed a reduced activation. Comparison of total (microscopic) and colony counts showed that only about 5% of unheated spores developed into colonies. This increased to about 50% after optimum heat activation.Keywords
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