Interference of GTP hydrolysis in the mechanism of microtubule assembly: an experimental study.
- 1 February 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (3), 771-775
- https://doi.org/10.1073/pnas.81.3.771
Abstract
The interference of GTP hydrolysis in the mechanism of microtubule assembly was studied, following the model and theory previously published. Results from dilution experiments show that microtubules depolymerize faster below the critical concentration than expected with a reversible polymerization model. The experimental plot of flux vs. tubulin concentration exhibits a slope discontinuity at the critical concentration, in agreement with the theory. Theoretical points calculated by the Monte Carlo method can be fitted qualitatively to the data. A consequence of this peculiar dynamic behavior of microtubules is that the ratio of tubulin dissociation and association rate constants measured, respectively, below and above the critical concentration does not yield the true value of the critical concentration. The presence of GTP at microtubule ends is necessary to maintain the stability of the polymer.This publication has 35 references indexed in Scilit:
- Mechanism of K+-induced actin assembly.The Journal of cell biology, 1982
- Fluorescence stopped‐flow study of the binding of S6‐GTP to tubulinFEBS Letters, 1982
- Assembly of microtubule protein: role of guanosine di- and triphosphate nucleotidesBiochemistry, 1982
- Implications of treadmilling for the stability and polarity of actin and tubulin polymers in vivo.The Journal of cell biology, 1980
- Characteristics of the polar assembly and disassembly of microtubules observed in vitro by darkfield light microscopy.The Journal of cell biology, 1979
- Outer doublet tubulin reassembly: evidence for opposite end assembly-disassembly at steady state and a disassembly end equilibriumBiochemistry, 1979
- Kinetic analysis of microtubule self-assembly in vitroJournal of Molecular Biology, 1977
- Neurotubule assembly at substoichiometric nucleotide levels using a GTP regenerating systemBiochemical and Biophysical Research Communications, 1977
- Head to tail polymerization of actinJournal of Molecular Biology, 1976
- Structural polarity and directional growth of microtubules of Chlamydomonas flagellaJournal of Molecular Biology, 1974