Fine structure of imbibed sclerotial cells ofClaviceps purpurea (Fr.)Tul revealed by freeze-etching
- 1 January 1978
- journal article
- Published by Wiley in Journal of Basic Microbiology
- Vol. 18 (2), 123-134
- https://doi.org/10.1002/jobm.3630180207
Abstract
The fine structure of the cortex of the natural sclerotium of Claviceps purpurea was studied. The cell wall of sclerotial cells is thickened due to overproduction of the fibrillar component of the wall. The intracellular spaces of the cortex tissue form a continuous system which is apparently instrumental in mediating communication between the growing sclerotium and the external milieu. The cytoplasmic membrane of imbibed sclerotial cells carriers abundant signs of secretory activity. Secretion vesicles, the content of which is discharged into external space, apparently contain exo 1,3-glucanase. Cytoplasmic vesicles migrating toward the plugged pores of the thickened septa apparently involve hydrolase secretion too. Spherosomes with lipid content are a predominant component of the cytoplasm of sclerotial cells. The activity of the membrane systems of imbibed cells indicates that the mobilization of lipids sets in only after activation of the hydrolases. Findings of phagocytosis of lipid granules by vacuoles are relatively frequent so that lipolysis might proceed in the vacuoles. Alkaloids could not be detected with the aid of freeze-etching.Keywords
This publication has 21 references indexed in Scilit:
- Metabolism of the Germinating Sclerotium of Claviceps purpureaJournal of General Microbiology, 1975
- The lipid globules of saccharomyces cerevisiae: A combined chemical and ultrastructural study using ultrathin frozen sectionsJournal of Ultrastructure Research, 1975
- Localization of alkaline phosphatase in Saccharomyces cerevisiae by means of ultrathin frozen sectionsJournal of Ultrastructure Research, 1975
- Metabolic Studies on Claviceps purpurea during Parasitic Development on RyeJournal of General Microbiology, 1974
- Autolysis of Extracellular Glucans Produced in vitro by a Strain of Claviceps fusiformisJournal of General Microbiology, 1970
- Fatty acid composition of sphacelial and sclerotial growth forms of Claviceps purpurea in relation to the production of ergoline alkaloids in cultureTransactions of the British Mycological Society, 1969
- Die Ultrastruktur der Apikalregion von PilzhyphenProtoplasma, 1969
- The Formation of Buds in YeastJournal of General Microbiology, 1969
- Water uptake, respiration pattern and lipid utilization in sclerotia of Claviceps purpurea during germinationTransactions of the British Mycological Society, 1968
- Cellular and extracellular structures in Cryptococcus laurentii and Rhodotorula speciesCanadian Journal of Microbiology, 1968