Magnetic resonance spectroscopy of normal and diseased muscles
- 1 December 1986
- journal article
- research article
- Published by Wiley in American Journal of Medical Genetics
- Vol. 25 (4), 659-679
- https://doi.org/10.1002/ajmg.1320250408
Abstract
Phosphorus magnetic resonance spectroscopy (P MRS) affords and innovative approach to the study of the oxidative enzyme content of normal and diseased muscles. Examples of the evaluation of the enzyme content of normal muscles by an exercise protoocol are provided. The protocol affords a hyperbolic work/cost profile, the Vmax of which is calculated by the reciprocal plots giving the enzyme content and the “effective Michaelis constant” with an evaluation of the resting metabolism. This steady state protocol clearly illustrates enzyme adaptation, on the one hand, and tissue atrophy particularly in the case of tissue injury, Duchenne's dystrophy, and genetic deletion of specific enzymes, on the other hand. The method is rapid, safe, and affords a quantitative evaluation of the disease process and possibilities for following appropriate therapies. So far, approx 1000 examinations or normal and diseased human limbs have been carried out in our laboratory in over the past four years.Keywords
This publication has 10 references indexed in Scilit:
- 31 P NMR studies in Duchenne muscular dystrophyNeurology, 1987
- Treatment of mitochondrial myopathy due to complex III deficiency with vitamins K3 and C: A 31P‐NMR follow‐up studyAnnals of Neurology, 1986
- AAEE News and CommentsMuscle & Nerve, 1986
- Control of oxidative metabolism and oxygen delivery in human skeletal muscle: a steady-state analysis of the work/energy cost transfer function.Proceedings of the National Academy of Sciences, 1985
- metabolic recovery after exercise and the assessment of mitochondrial function in Vivo in human skeletal muscle by means of 31P NMRMagnetic Resonance in Medicine, 1984
- 31P NMR study of improvement in oxidative phosphorylation by vitamins K3 and C in a patient with a defect in electron transport at complex III in skeletal muscle.Proceedings of the National Academy of Sciences, 1984
- 31P NMR studies of control of mitochondrial function in phosphofructokinase-deficient human skeletal muscle.Proceedings of the National Academy of Sciences, 1982
- Examination of a Case of Suspected McArdle's Syndrome by31P Nuclear Magnetic ResonanceNew England Journal of Medicine, 1981
- Computed Medical ImagingScience, 1980
- RESPIRATORY ENZYMES IN OXIDATIVE PHOSPHORYLATIONJournal of Biological Chemistry, 1955