The Localized Hemolysis-in-Gel Method Adapted to the Detection of Spleen Cells Releasing Virus-Specific Antibodies

Abstract

The localized hemolysis-in-gel method has been adapted to the detection of single cells releasing antibodies to influenza A virus. In mice infected with influenza A the number of cells releasing IgM antibodies specific for the inner component of the virus (RNP antigen) begins to rise between 24 and 36 hours after virus inoculation, reaches a maximum on the 4th day, and then decreases rapidly to the background level by day 20.