Variable expression of the ssb-1 allele in different strains of Escherichia coli K12 and B: Differential suppression of its effects on DNA replication, DNA repair and ultraviolet mutagenesis
- 1 October 1981
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 183 (2), 348-355
- https://doi.org/10.1007/bf00270639
Abstract
Summary We have transduced the mutant allele ssb-1, which encodes a temperature-sensitive single-strand DNA binding protein (SSB), into several Escherichia coli strains, and have examined colony-forming ability, DNA replication, sensitivity to ultraviolet light (UV) and UV-induced mutability at the nonpermissive temperature. We have found: 1) that the degree of ssb-1-mediated temperature-sensitivity of colony-forming ability and of DNA replication is strain-dependent, resulting in plating efficiencies at 42° C (relative to 30° C) ranging from 100% to 0.002%; 2) that complete suppression of the temperature-sensitivity caused by ssb-1 occurs only on nutrient agar, and not in any other medium tested; 3) that strains in which ssb-1-mediated temperature-sensitivity is completely suppressed show moderate UV sensitivity and normal UV mutability at 30° C, but much more extreme UV sensitivity and drastically reduced UV mutability at 42° C; and 4) that defects in excision repair or in other Uvr+-dependent processes are not responsible for most of the UV sensitivity promoted by ssb-1. We discuss our results in relation to the known properties of SSB and its possible role in the induction of DNA damage-inducible (SOS) functions.Keywords
This publication has 47 references indexed in Scilit:
- E. coli recA protein-directed cleavage of phage λ repressor requires polynucleotideNature, 1980
- Fine structure mapping and properties of mutations suppressing thelonmutation inEscherichia coliK-12 and B strainsGenetics Research, 1977
- Partial suppression of the LexA phenotype by mutations (rnm) which restore ultraviolet resistance but not ultraviolet mulability to Escherichia coli B/r uvrA lexAMutation Research, 1976
- Mutation frequency decline for streptomycin - resistant mutations induced by ultraviolet light in Escherichia coli B/rMutation Research, 1972
- Exchanges between DNA strands in ultraviolet-irradiated Escherichia coliJournal of Molecular Biology, 1971
- ULTRAVIOLET-INDUCED MUTATION AND DNA REPAIRAnnual Review of Microbiology, 1969
- ACTION SPECTRA FOR PHOTOREACTIVATION OF KILLING AND MUTATION TO PROTOTROPHY IN U.V.‐SENSITIVE STRAINS OF ESCHERICHIA COLI POSSESSING AND LACKING PHOTOREACTIVATING ENZYME*Photochemistry and Photobiology, 1966
- Radiation-Induced Mutations and Their RepairScience, 1966
- ULTRAVIOLET‐INDUCED LETHALITY AND REVERSION TO PROTOTROPHY IN ESCHERICHIA COLI STRAINS WITH NORMAL AND REDUCED DARK REPAIR ABILITYPhotochemistry and Photobiology, 1965
- Photoreversal and “dark repair” of mutations to prototrophy induced by ultraviolet light in photoreactivable and non-photoreactivable strains of Escherichia coliMutation Research, 1964