Inheritance and linkage of isozymes in Populus tremuloides (Michx.)

Abstract

Progenies of four controlled crosses were assayed electrophoretically to determine the inheritance of isozymes of 10 loci coding for six enzymes, aconitase (ACO), glutamate oxaloacetate transaminase (GOT), isocitrate dehydrogenase (IDH), phosphoglucomutase (PGM), 6-phosphogluconate dehydrogenase (6-PGD), and phosphoglucose isomerase (PGI), in roots of Populus tremuloides. Chi-square goodness of fit tests verified a single-gene Mendelian control of the segregating allozyme variants at each of five loci: Aco-1, Got, Pgm-2, 6-Pgd-2, and Pgi-2. Evidence was also obtained for a single-gene control of each of the remaining five loci (Aco-2, Idh, Pgm-1, 6-Pgd-1, and Pgi-1). ACO and PGM showed monomeric, while GOT, IDH, 6-PGD, and PGI had dimeric, banding patterns. The results of joint two-locus segregation tests indicated no linkage between 6-Pgd-2 and Pgi-2. Key words: Populus species, electrophoresis, allozymes, inheritance, linkage.