The nucteotide sequence of myodn light chain (L-2A) mRNA from embryonic chicken cardiac muscle tissue

Abstract

The nucleotide sequence of a cDNA clone (pML10) for chicken cardiac myosin light chain is described. The cDNA insert contains 613 nucleotides representing the entire coding sequence, with the exception of nine NH₂–tenrnnal ammo acids, and the full 3′non-coding region of 146 nucleotides. The missing 5′ terminus of the mRNA, not represented in the clone p^ML10, was obtained by extension of the cDNA using a 43 nucleotide long internal EcoRI fragment as a primer. The non-coding region contains several direct and inverted repeated sequences and the polyadenylation signal sequence AATAAA. The coding portion exhibits non-random usage of synonymous codons with a strong bias for codons ending in G and C.