Identification of Genes Involved in Cytochrome c Biogenesis in Shewanella oneidensis , Using a Modified mariner Transposon
Open Access
- 1 August 2005
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 71 (8), 4935-4937
- https://doi.org/10.1128/aem.71.8.4935-4937.2005
Abstract
A modified mariner transposon, miniHimar RB1, was generated to mutagenize cells of the metal-reducing bacterium Shewanella oneidensis. The use of this transposon led to the isolation of stable mutants and allowed rapid identification of disrupted genes. Fifty-eight mutants, including BG104 and BG148 with transposon insertions in the cytochrome c maturation genes ccmC and ccmF1, respectively, were analyzed. Both mutants were deficient in anaerobic respiration and cytochrome c production.Keywords
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