High efficiency temperature-sensitive amber suppressor strains of Escherichia coli K12
- 1 May 1980
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 178 (2), 293-299
- https://doi.org/10.1007/bf00270475
Abstract
A set of eight strains combining the supD43,74 ts1 suppressor gene with alleles of three suppressor-enhancing (sue) genes have been constructed and characterized. The sue mutations work cooperatively to raise suppressor activity and together raise the activity of the supD43, 74-encoded suppressor 40-fold. These strains further expand the utility of the ts suppressor system by providing as much as 100% suppressor activity at temperatures at or below 20°C to as little as 0.015% suppressor activity at 43°C.This publication has 21 references indexed in Scilit:
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