Detection of colt serum antibody against Corynebacterium equi by agar gel diffusion.

Abstract

Agar gel diffusion was applied to detect colt serum antibody against C. equi. Concentrated culture supernates and bacterial sonicates prepared from 9 strains of C. equi were used as antigens. The presence of precipitating antibodies was demonstrated in the sera of all affected colts, but not at all in those of the healthy colts examined. Precipitating lines were observed between both antigens and affected colt sera. Precipitating antibodies against concentrated culture supernates appeared only in the 7S globulin fraction. One precipitating line against bacterial sonicates was demonstrated in the 7S globulin fraction and the other in both 7S and 19S globulin fractions separated with Sephadex G-200 column chromatography.