Immunologic Studies of Rabbit Bone-Marrow with the Aid of Gel Diffusion and Biologic Techniques

Abstract

Hyperimmune serum to rabbit bone-marrow was produced in horses. Several serologically active factors were demonstrated in this sera. Using a modification of Ouchterony''s gel diffusion technique, 5 antigens were found in rabbit bone-marrow (A,B, Bl,C, D). Analysis of organs revealed that the spleen contains A,B,C, the liver B1,C,D, the lung B1,C,D, and the adrenals B1,C. Using specific absorption various antisera were produced. Anti-B serum caused anemia, anisocytosis and polychromasia but did not affect platelets or white cells. Anti-AB serum caused hemorrhage in the adrenal cortex, lymphocytosis, lymphadenosis, high level of nucleated red cells and hypothermia. Anti-ABC serum caused anemia, reticulocytosis, anisocytosis, red ceil basophilia, decrease in white cells and thrombocytes and lowering of body temperature. Complete anti-bone marrow serum caused acute shock and changes similar to those following injection of anti-ABCD serum except no basophilia of red blood cells. Anti-bone marrow serum saturated with plasma and bone marrow caused moderate anemia and anisocytosis.