Three‐dimensional structure of the higher‐plant photosystem II reaction centre and evidence for its dimeric organization in vivo
- 1 April 1994
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 221 (1), 307-315
- https://doi.org/10.1111/j.1432-1033.1994.tb18742.x
Abstract
The three-dimensional structure of photosystem II (PSII) has been determined by conventional transmission electron microscopy and computerized three-dimensional reconstruction. Both the complete system and that lacking the oxygen-evolving complex have been analyzed. The PSII complex has a four-lobed structure with twofold symmetry. An estimate of the molecular mass and the results of Deriphat/PAGE analysis suggest that a reaction centre is present in each half of the structure resolved by electron microscopy. Stepwise removal of components of the complex showed that the removal of CP47 (a 47-kDa chlorophyll-protein complex) induces monomerization of PSII, which indicates the importance of this subunit for the dimeric structure.Keywords
This publication has 36 references indexed in Scilit:
- Three-dimensional structure of photosystem IINature, 1993
- A supramolecular light‐harvesting complex from chloroplast photosystem‐II membranesEuropean Journal of Biochemistry, 1992
- CHLOROPHYLL BINDING PROTEINS WITH ANTENNA FUNCTION IN HIGHER PLANTS and GREEN ALGAEPhotochemistry and Photobiology, 1990
- Functional and structural analysis of photosystem II core complexes from spinach with high oxygen evolution capacityEuropean Journal of Biochemistry, 1990
- Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopyBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1989
- Identification of a new gene in the chloroplast genome encoding a low‐molecular‐mass polypeptide of photosystem II complexFEBS Letters, 1988
- Similarity measures between imagesUltramicroscopy, 1987
- Three-dimensional structure of the surface layer protein of Clostridium thermohydrosulfuricumJournal of Ultrastructure and Molecular Structure Research, 1986
- A highly resolved, oxygen‐evolving photosystem II preparation from spinach thylakoid membranesFEBS Letters, 1981
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970