Interactions between the Su(Hw) and Mod(mdg4) proteins required for gypsy insulator function
Open Access
- 15 May 2001
- journal article
- research article
- Published by Springer Nature in The EMBO Journal
- Vol. 20 (10), 2518-2527
- https://doi.org/10.1093/emboj/20.10.2518
Abstract
The gypsy insulator is thought to play a role in nuclear organization and the establishment of higher order chromatin domains by bringing together several individual insulator sites to form rosette‐like structures in the interphase nucleus. The Su(Hw) and Mod(mdg4) proteins are components of the gypsy insulator required for its effect on enhancer–promoter interactions. Using the yeast two‐hybrid system, we show that the Mod(mdg4) protein can form homodimers, which can then interact with Su(Hw). The BTB domain of Mod(mdg4) is involved in homodimerization, whereas the C‐terminal region of the protein is involved in interactions with the leucine zipper and adjacent regions of the Su(Hw) protein. Analyses using immunolocalization on polytene chromosomes confirm the involvement of these domains in mediating the interactions between these proteins. Studies using diploid interphase cells further suggest the contribution of these domains to the formation of rosette‐like structures in the nucleus. The results provide a biochemical basis for the aggregation of multiple insulator sites and support the role of the gypsy insulator in nuclear organization.Keywords
This publication has 35 references indexed in Scilit:
- Protein encoding by both DNA strandsNature, 2001
- Loss of Insulator Activity by Paired Su(Hw) Chromatin InsulatorsScience, 2001
- Effects of cis Arrangement of Chromatin Insulators on Enhancer-Blocking ActivityScience, 2001
- Insulators and Boundaries: Versatile Regulatory Elements in the Eukaryotic GenomeScience, 2001
- Stopped at the border: boundaries and insulatorsCurrent Opinion in Genetics & Development, 1999
- Co-operative DNA binding by GAGA transcription factor requires the conserved BTB/POZ domain and reorganizes promoter topologyThe EMBO Journal, 1999
- Fab-7 functions as a chromatin domain boundary to ensure proper segment specification by the Drosophila bithorax complex.Genes & Development, 1996
- The Fab-7 element of the bithorax complex attenuates enhancer-promoter interactions in the Drosophila embryo.Genes & Development, 1996
- A 5′ element of the chicken β-globin domain serves as an insulator in human erythroid cells and protects against position effect in DrosophilaCell, 1993
- A position-effect assay for boundaries of higher order chromosomal domainsCell, 1991