Presence of human immunodeficiency virus nucleic acids in wastewater and their detection by polymerase chain reaction
- 1 December 1992
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 58 (12), 3984-3990
- https://doi.org/10.1128/aem.58.12.3984-3990.1992
Abstract
The human immunodeficiency virus type 1 (HIV-1) released by infected individuals or present in human and hospital wastes can potentially cause contamination problems. The presence of HIV-1 was investigated in 16 environmental samples, including raw wastewater, sludge, final effluent, soil, and pond water, collected from different locations. A method was developed to extract total nucleic acids in intact form directly from the raw samples or from the viral concentrates of the raw samples. The isolated nucleic acids were analyzed for the presence of HIV-1 by using in vitro amplification of the target sequences by the polymerase chain reaction (PCR) method. HIV-1-specific proviral DNA and viral RNA were detected in the extracted nucleic acids obtained from three wastewater samples by this method. The specificity of the PCR-amplified products was determined by Southern blot hybridization with an HIV-1-specific oligonucleotide probe, SK19. The isolated nucleic acids from wastewater samples were also screened for the presence of poliovirus type 1, representing a commonly found enteric virus, and simian immunodeficiency virus, representing, presumably, rare viruses. While poliovirus type 1 viral RNA was found in all of the wastewater samples, none of the samples yielded a simian immunodeficiency virus-specific product. No PCR-amplified product was yielded when wastewater samples were directly used for the detection of HIV-1 and poliovirus type 1. The wastewater constituents appeared to be inhibitory to the enzymes reverse transcriptase and DNA polymerase.(ABSTRACT TRUNCATED AT 250 WORDS) ImagesKeywords
This publication has 23 references indexed in Scilit:
- Persistent Diarrhea and Fecal Shedding of Retroviral Nucleic Acids in Children Infected with Human Immunodeficiency VirusThe Journal of Infectious Diseases, 1991
- Recovery of Human Immunodeficiency Virus Type 1 from Semen: Minimal Impact of Stage of Infection and Current Antiviral ChemotherapyThe Journal of Infectious Diseases, 1991
- ISOLATION OF AIDS-ASSOCIATED RETROVIRUS FROM GENITAL SECRETIONS OF WOMEN WITH ANTIBODIES TO THE VIRUSThe Lancet, 1986
- HTLV-III Exposure during Cardiopulmonary ResuscitationNew England Journal of Medicine, 1985
- ISOLATION OF AIDS VIRUS FROM CELL-FREE BREAST MILK OF THREE HEALTHY VIRUS CARRIERSThe Lancet, 1985
- ISOLATION OF HUMAN T-LYMPHOTROPIC VIRUS TYPE III FROM THE TEARS OF A PATIENT WITH THE ACQUIRED IMMUNODEFICIENCY SYNDROMEThe Lancet, 1985
- Transplacental Transmission of HTLV-III VirusNew England Journal of Medicine, 1985
- HTLV-III in Saliva of People with AIDS-Related Complex and Healthy Homosexual Men at Risk for AIDSScience, 1984
- Isolation of Lymphocytopathic Retroviruses from San Francisco Patients with AIDSScience, 1984
- Time-dependent effects of Metrazol on memoryPharmacology Biochemistry and Behavior, 1973