Glycerokinase in human adipose tissue
- 1 March 1977
- Vol. 12 (3), 272-277
- https://doi.org/10.1007/bf02533346
Abstract
The presence of glycerokinase has been demonstrated in human omental and subcutaneous adipose tissue. The enzyme reaction showed a linear time course for 5 min at 30 C and pH optima at pH 7.6 and 9.0. Saturation of the enzyme was observed at 1.8 mM adenosine triphosphate (ATP) and the double reciprocal plot of activity vs. ATP concentration was nonlinear giving two apparent Km values of 0.094 and 0.518 mM. The apparent Km for glycerol, 0.112 mM, was obtained from a linear double reciprocal plot, and the enzyme was saturated at about 0.4 mM glycerol. The activity of glycerokinase in human adipose tissue excised under general anaesthesia was low and was unrelated to adipose cell size or the degree of obesity of the subject from whom the fat was obtained.This publication has 29 references indexed in Scilit:
- Effects of overfeeding on lipid and carbohydrate metabolism in lean young adultsMetabolism, 1972
- THE EFFECT OF MATERNAL MALNUTRITION ON THE PROGENY IN THE RATImmunology & Cell Biology, 1972
- Regulation of glycerol kinase by insulin in isolated fat cells and liver of Bar Harbor Obese MiceDiabetologia, 1971
- Glycerin-Kinase und Lipolyse des menschlichen Fettgewebes in Abhängigkeit vom relativen KörpergewichtHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1971
- Lipolysis and lipogenesis from glucose in human fat cells of different sizesJCI Insight, 1970
- Glycerol Kinase Activity in Isolated Fat Cells of Bhob MicEHormone and Metabolic Research, 1970
- Kinetics of Glycerol Kinases from Mammalian Liver and Candida mycodermaEuropean Journal of Biochemistry, 1967
- A radiochemical enzymatic activity assay for glycerol kinase and hexokinaseBiochimica et Biophysica Acta (BBA) - Enzymology, 1967
- A Rapid Enzymatic Assay for GlycerolNature, 1962
- PAPER CHROMATOGRAPHIC SEPARATION OF SOME: BIOLOGICALLY IMPORTANT PHOSPHATE ESTERSCanadian Journal of Chemistry, 1952