Repression of nitrogen fixation in Klebsiella pneumoniae at high temperature

Abstract
The effect of elevated growth temperature on nif mRNA synthesis, nif protein synthesis and nitrogenase activity was investigated in wild-type and NifL- strains of K. pneumoniae. Nitrogenase activity is not affected at 41.degree. C; however, nitrogenase is not synthesized at that temperature. Transcription of 3 nif operons studied is repressed at 41.degree. C. Apparently, the nifL protein is not required for repression by high temperature and repression of nif at 41.degree. C results from a reversible inactivation of the nifA gene product.