Gene expression of an Escherichia coli ribosomal RNA promoter fused to structural genes of the galactose operon.
- 1 November 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (11), 5799-5803
- https://doi.org/10.1073/pnas.76.11.5799
Abstract
The promoter region of the rrnB rRNA gene of E. coli was ligated within the epimerase gene (galE) of the galactose operon in a .lambda. phage vector. The recombinant .lambda. phage was characterized by restriction mapping and assays of both galK (galactokinase) gene activity and galactose mRNA hybridization. In such lysogens, expression of the fused galactose operon occurs as a function of growth rate in a manner characteristic of rRNA gene expression and is subject to stringent control by amino acid availability for protein synthesis. Galactose mRNA arising from the ribosomal promoter is not as metabolically stable as rRNA.This publication has 22 references indexed in Scilit:
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