The Use of 1-Anilino-8-naphthalene Sulfonate as Fluorescent Probe for Conformational Studies on Ribulose-1,5-bisphosphate Carboxylase
Open Access
- 1 June 1976
- journal article
- research article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung C
- Vol. 31 (5-6), 267-271
- https://doi.org/10.1515/znc-1976-5-609
Abstract
The influence of Mg2+ ions and temperature on the structure of the enzyme ribulose-1,5-bisphosphate carboxylase from spinach was investigated using the fluorescent probe 1-anilino-8-naphthalene sulfonate (ANS). The binding of ANS to the enzyme molecule caused a significant increase of fluorescence emission which was further enhanced by the addition of Mg2+. The temperature dependence of the fluorescence emission indicated a conformational change of the enzyme between 12 and 24.degree. C. The Mg2+ and the temperature effects were additive. ANS itself did not change the conformation of the enzyme. The influence of the substrates CO2 and ribulose-1,5-bisphosphate, and the effect of the pH of the medium and of a sulfhydryl reducing reagent on fluorescence emission were analyzed.Keywords
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