DETERMINATION OF MICRO QUANTITIES OF ANDROSTERONE, AETIOCHOLANOLONE AND DEHYDROEPIANDROSTERONE BY GAS-LIQUID CHROMATOGRAPHY

Abstract

A convenient, sensitive and reproducible method for the determination of androsterone, aetiocholanolone and dehydroepiandrosterone, as their trimethylsilyl ether derivatives, has been described. The conditions include: 3.3 % XE-60 on Anakrom ABS (90-100 mesh), 6 ft. × 3.8 mm i. d. stainless steel column, 225° column temperature, 327° vaporizer temperature, nitrogen pressure 11.0 p. s. i. (34 ml/min flow). The % variation between duplicate samples for androsterone, aetiocholanolone and dehydroepiandrosterone respectively was 1.5, 4.5 and 1.8 at the 0.3 μg level. Quantitation at the 0.05 μg level appears possible.