The Spatiotemporal Analysis of Odorants at the Level of the Olfactory Receptor Sheet

Abstract

Activity in 2 separate regions of the frog olfactory mucosa was sampled by simultaneously recording the summated neural discharges from the olfactory nerve branches originating from them. The difference in the activity from these 2 regions in response to a stimulus was measured by the ratio of the response amplitude recorded from the lateral nerve branch to that recorded from the medial nerve branch (LB/MB ratio), the latency difference (or time interval) between these 2 responses. Equal concentrations of 4 different odorants were drawn into the nose by an artificially produced sniff of known dimensions. At each concentration in every animal the four chemicals were ranked in order of the magnitudes of their LB/MB ratios and again in order of their latency differences. Regardless of their concentration, the same chemicals fell into the same ranks in different animals. In addition, for each chemical the magnitudes of the ratios and latency differences showed only minimal changes with concentration. Thus, spatiotemporal patterns of relative response magnitude and latency differences across the mucosa differentially represented the odorants. Such a spatiotemporal code, together with physicochemical considerations, suggested that the nose separates vapors in a manner similar to a gas chromatograph. This is further supported by the previously observed reversal of the ratio patterns with reversal of air flow direction through the olfactory sac.