HEMOLYSIS OF RABBIT ERYTHROCYTES BY PURIFIED STAPHYLOCOCCAL ALPHA-TOXIN III
Open Access
- 1 January 1964
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 87 (1), 145-149
- https://doi.org/10.1128/jb.87.1.145-149.1964
Abstract
Madoff, Morton A. (New England Center Hospital, Boston, Mass.), Louis Z. Cooper, and Louis Weinstein. Hemolysis of rabbit erythrocytes by purified staphylococcal alpha-toxin. III. Potassium release. J. Bacteriol. 87:145–149. 1964.—The reaction between staphylococcal alpha-toxin and erythrocytes was characterized by rapid release of K+ from the cells, early in the prelytic period; 50 to 75% of this loss occurred before leakage of hemoglobin was detectable. The addition of specific antitoxin early enough in the reaction to inhibit gross hemolysis also inhibited cation release. The presence of sucrose or polyethylene glycol prevented hemoglobin release, but was without effect on K+ leak. These observations suggest that K+ loss is a more specific indication of the progress of the reaction between alpha-toxin and erythrocytes than is the release of hemoglobin.Keywords
This publication has 13 references indexed in Scilit:
- HEMOLYSIS OF RABBIT ERYTHROCYTES BY PURIFIED STAPHYLOCOCCAL ALPHA-TOXIN IIJournal of Bacteriology, 1964
- HEMOLYSIS OF RABBIT ERYTHROCYTES BY PURIFIED STAPHYLOCOCCAL ALPHA-TOXIN IJournal of Bacteriology, 1964
- Cation Transport and Structure of the Red-Cell Plasma MembraneCirculation, 1962
- EFFECTS OF SULFHYDRYL INHIBITION ON RED BLOOD CELLS. I. MECHANISM OF HEMOLYSIS*JCI Insight, 1962
- EFFECT OF ANTIBODY AND COMPLEMENT ON PERMEABILITY CONTROL IN ASCITES TUMOR CELLS AND ERYTHROCYTESThe Journal of Experimental Medicine, 1959
- Sodium and potassium movements in human red cellsThe Journal of Physiology, 1956
- THE PERMEABILITY OF THE HUMAN ERYTHROCYTE TO SODIUM AND POTASSIUMThe Journal of general physiology, 1952
- Factors in the active transport of cationsThe Journal of Physiology, 1951
- COMPARATIVE KINETICS OF HEMOLYSIS INDUCED BY BACTERIAL AND OTHER HEMOLYSINSThe Journal of general physiology, 1947
- Studies on the permeability of erythrocytesBiochemical Journal, 1938