Propagation in vitro of Polioviruses. VIII. Effect of pH on Virus Yield and Cell Metabolism.

Abstract
The pH of tissue culture medium was altered purposefully to measure quantitatively the effects upon virus yield and host cell metabolism. By use of strain HeLa cells and poliovirus Type 1 (strain Mahoney) for growth in media of pH from 6.3 to 8.0 it was established that virus yield was affected by the pH of the culture medium. Decreased virus yield at decreased or increased pH levels was related to a decrease in metabolic rate of host cell cultures.