The isolation of mouse antigens carrying H2-histocompatibility specificity: some preliminary studies

Abstract

Ascitic fluid, induced in mice by tumor cells, was used as a source of antigens carrying H2-histocompatibility specificity. Activity, measured by inhibition of hemagglutination induced in mouse anti-mouse sera with dextran and normal human serum, shows a 500-1000-fold increase on purification. The product is a lipoprotein, in which only 1-2% of impurity has been detected although a variety of tests have been used. The substance is insoluble and is believed to be a structural component of cell membranes. Material isolated from different inbred mouse strains carry the H2 specificities expected from the H[image] antigenic characters associated with these mouse strains. Analogous material isolated from non-specific tumor-cell products grown in inbred mice do not carry the specificities of that mouse. No analogous substance is present in normal serum.