Role of the host in virus assembly: cloning of the Escherichia coli groE gene and identification of its protein product.
- 1 January 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (1), 136-139
- https://doi.org/10.1073/pnas.75.1.136
Abstract
Correct assembly of the heads of bacteriophages .lambda. and T4 requires the function of the groE gene of the E. coli host. A transducing derivative of .lambda., called .lambda.gt-Ec .cntdot. groE, that carries a functional copy of the groE gene was isolated. Unlike wild-type .lambda., this phage can form plaques on hosts with a mutant groE gene. An amber mutation in the groE gene carried by the phage was isolated, and this identified the groE product as a protein of MW 65,000. In the phage, the groE gene is under the control of an early phage promoter.This publication has 14 references indexed in Scilit:
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