Three‐Step Purification of Glucocorticoid Receptors from Rat Liver
Open Access
- 1 July 1980
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 108 (1), 47-53
- https://doi.org/10.1111/j.1432-1033.1980.tb04694.x
Abstract
The 90000-Mr glucocorticoid receptor was purified to homogeneity according to sodium dodecyl-sulfate/polyacrylamide gel electrophoresis. An affinity column containing either dexamethasone-17β-carboxylic acid or dexamethasone-21-methanesulfonate bound to the matrix with the help of a disulfide bond is used in this study. Using this affinity matrix, in a single step, 8700-fold purification of glucocorticoid receptor from rat liver cytosol could be achieved. Following the method of activation and DNA-cellulose chromatography the 90000-Mr receptor subunit was purified to homogeneity.This publication has 37 references indexed in Scilit:
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