CHEMICAL, CLINICAL, AND IMMUNOLOGICAL STUDIES ON THE PRODUCTS OF HUMAN PLASMA FRACTIONATION. II. ELECTROPHORETIC AND ULTRACENTRIFUGAL STUDIES OF SOLUTIONS OF HUMAN SERUM ALBUMIN AND IMMUNE SERUM GLOBULINS 12

Abstract

Results of electrophoretic and ultracentrifugal analyses on serum albumin solns. indicated that fibrinogen, [beta]-, and [gamma]-globulin, and components of molecular wt. as large or larger than the normal globulins of plasma, are not present within the accuracy of these methods of analysis. The electrophoretic analyses were carried out on 162 preps. delivered to the armed forces by 7 different laboratories and indicate that the albumin is routinely concentrated by this method of fractionation from a value of 55 or 60% in plasma to a value of 98.5%. The immune serum globulins of 35 preps. from 4 laboratories were studied and indicate that the 7-globulin content of these materials is increased from about 11% in plasma to about 87%, and, in most of the more recent preps., to over 95%, the main impurities being B-globulin and albumin. An avg. value of very nearly 20% of fast-moving material, in large part 7-globulin, was observed in the ultracentrifuge, the amt. of this material being quite uniform in nearly all preps. These studies give evidence of the reproducibility of these materials, and provide the chemical specification of purity used in setting up minimum requirements for the acceptance for the armed forces of these products of plasma fractionation.