Junction between Z and B conformations in a DNA restriction fragment: evaluation by Raman spectroscopy.
- 1 April 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (8), 2549-2553
- https://doi.org/10.1073/pnas.79.8.2549
Abstract
Raman vibrational spectra were obtained from 2 DNA restriction fragments and the DNA polymer (dG-dC)n.cntdot.(dG-dC)n in 0.01 and 4.5 M NaCl. One fragment contained 95 base pairs (bp) of the Escherichia coli lactose operator-promoter region (95 bp fragment). The other fragment consisted of the 95 bp region flanked by 26 and 32 bp of dC-dG sequences and BamHI ends (157-bp fragment). In 0.01 M NaCl all 3 DNA species have Raman spectra characteristic of a right-handed B conformation. The high salt spectrum of the 95 bp fragment is also characteristic of a B conformation. The spectrum of the 157 bp fragment in 4.5 M NaCl shows major intensity changes from the 0.01 M NaCl spectrum. These changes are also observed in the high salt spectra of (dG-dC)n.cntdot.(dG-dC)n and are correlated with the presence of a left-handed Z conformation. Comparisons between the high salt Raman spectra of the 157 bp fragment indicated that essentially all of the dC-dG regions in the 157 bp fragment are in the Z conformation and a large part (.apprx. 80%) of the 95 bp region no longer has a B-type backbone vibration. However, this non-B-DNA-like character of the central region is not indicated by base vibrations.This publication has 35 references indexed in Scilit:
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