We have studied the presence and state of human papillomavirus (HPV) genomes in condylomata acuminata of the vulva, vagina, and cervix and in flat condylomas of the cervix from Japanese females by DNA hybridization with HPV 6 and 11 DNAs. Unintegrated HPV 6-related DNA (HPV 6 or 11 DNA) was detected in 55 out of 60 condylomata acuminata and in 1 out of 4 flat condylomas under stringent hybridization conditions. The HPV DNAs were identified as HPV 6 DNA in 29 condylomata acuminata (20 vulvar, 3 vaginal, and 6 cervical samples) and 1 flat condyloma and as HPV 11 DNA in 6 condylomata acuminata (6 vulvar samples). The data indicate close association of HPV types 6 and 11 with condylomata.