RNA synthesis by nuclei and nucleoli from ischemic liver cells
- 1 December 1978
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 97 (3), 487-496
- https://doi.org/10.1002/jcp.1040970324
Abstract
Nuclei and nucleoli were isolated from rat livers subjected to an interruption of the blood supply for periods of different duration, as well as after restoration of the blood supply. They were assayed for RNA synthesis under conditions of diverse ionic strengths, and in the presence of an exogenous template, such as poly d (A–T), and actinomycin to inactivate the endogenous template; α-amanitin was made used of to distinguish polymerase I and polymerase II dependent RNA synthesis. Nuclei and nucleoli from ischemic livers showed a severe impairment of RNA synthesis, which is likely to be due to decreased initiation frequency of the engaged polymerases, while free polymerases were essentially unchanged. Both form I and II polymerase were equally involved. After restoration of the blood supply RNA synthesis recovered with an overshooting well above normal levels of activity, lasting for at least 24 hours. Increased RNA synthesis was not followed by thymidine incorporation into DNA.This publication has 44 references indexed in Scilit:
- Nucleoli of thioacetamide-treated liver as a model for studying regulation of preribosomal RNA synthesisBiochemical and Biophysical Research Communications, 1977
- Rapid increase in poly(A) (+) mRNA content following inhibition of protein synthesisJournal of Cellular Physiology, 1977
- Effect of the ionic environment on the transcriptional activity of rat liver nucleoliExperimental Cell Research, 1976
- Control of nucleolar RNA synthesis by the intracellular pool sizes of ATP and GTPCell, 1976
- Two functional states of the RNA polymerases in the rat hepatic nuclear and nucleolar fractionsNature, 1974
- Diurnal rhythmicity of mammalian DNA-dependent RNA polymerase activities I and II: Dependence on food intakeBiochemical and Biophysical Research Communications, 1973
- Transcription of ribonucleic acid in isolated mouse myeloma nucleiBiochemistry, 1973
- Ribosomal RNA synthesis in isolated nucleiJournal of Molecular Biology, 1972
- Ischemic effects on kidney nuclear RNA synthesisLife Sciences, 1970
- Kinetics of the in vitro inhibition of transcription by actinomycinJournal of Molecular Biology, 1970