DISPROPORTIONAL IMMUNOSTAINING PATTERNS OF 2 SECRETORY PROTEINS IN GUINEA-PIG AND RAT EXOCRINE PANCREATIC-CELLS - AN IMMUNOFERRITIN AND FLUORESCENCE STUDY

Abstract

Amylase (Am) and chymotrypsinogen (Chtg) were demonstrated in rat and guinea pig exocrine pancreastic cells by immunofluorescence and immunoferritin cytochemistry on thin and ultrathin frozen sections. Two observations indicate that Am and Chtg may behave differently in the pre-Golgi phase of their intracellular transport. Aggregates of material within the RER [rough endoplasmic reticulum] cisternae of the guinea pig (so-called intracisternal granules) reacted strongly with anti-Chtg, but showed no affinity for anti-Am. In both rat and guinea pig, the increase in labeling intensity from cytoplasm (RER) to secretory granules was larger for Chtg than for Am. The two proteins may not travel in-parallel towards the Golgi complex. Compared with Chtg, Am would lag behind in the RER cisternae.