Insoluble antibody column for isolation and quantitative determination of gibberellins

Abstract

A column that specifically binds gibberellins was prepared by covalent binding of anti-GA antibodies to Sepharose. By using such an immunoadsorbent column, radio-immunoassay for quantitative determination of gibberellins was developed. Using this method, diffusible GA from apices of etiolated pea seedlings was isolated in one step and determined quantitatively.