The Covalent Structure of Calf Skin Type III Collagen. I. The Amino Acid Sequence of the Amino Terminal Region of the αl(III) Chain (Position 1-222)

Abstract

The amino terminal 227 amino acid residues of the .alpha.1(III) chain contain 4 CNBr peptides: .alpha.1(III)CB3A (79 residues), CB3B, CB3C (6 residues each), CB7 (37 residues) and CB6 (99 residues). Fragmentation of the CNBr peptides was carried out using trypsin [EC 3.4.21.4], chymotrypsin [EC 3.4.21.1] and the protease from Staphylococcus aureus V8. The fragments obtained were isolated by a combination of molecular sieve and ion exchange chromatography. The sequence analysis was performed according to the automated Edman degradation procedure.