Kinetic evidence for hapten-induced conformational transition in immunoglobin MOPC 460.
- 1 October 1976
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (10), 3549-3553
- https://doi.org/10.1073/pnas.73.10.3549
Abstract
The kinetics of hapten binding to the homogeneous immunoglobulin A secreted by the murine plasmacytoma MOPC 460 was investigated by the chemical relaxation method. Two distinct relaxation times were observed in the binding equilibrium with 3 different haptens. A detailed concentration dependence analysis of relaxation times and amplitudes was performed with the hapten .epsilon.-N-(2,4-dinitrophenyl)-lysine (Dnp-Lys). Two interconvertible conformational states of the protein apparently bind the hapten with different association constants. Hapten binding shifts the equilibrium towards the better binding state. These observations form kinetic evidence for a conformational transition induced in the immunoglobulin by ligand binding to its antigen binding site, and support the allosteric hypothesis for the initiation of physiological functions by antigen-antibody association.This publication has 15 references indexed in Scilit:
- Activation of antibody Fc function by antigen-induced conformational changes.Proceedings of the National Academy of Sciences, 1975
- Antigen-induced conformational changes in antibodies and their Fab fragments studied by circular polarization of fluorescence.Proceedings of the National Academy of Sciences, 1975
- Shape and volume of fragments Fab' and (Fab')2 of anti-poly(D-alanyl) antibodies in the presence and absence of tetra-D-alanine as determined by small-angle x-ray scatteringBiochemistry, 1975
- Kinetics of antibody--hapten reactions.1975
- On the analysis of fluorescence decay kinetics by the method of least-squaresAnalytical Biochemistry, 1974
- Effect of Antigen Binding on the Properties of AntibodyAdvances in Immunology, 1974
- Contact Regions for Dinitrophenyl and Menadione Haptens in an Immunoglobulin Binding More Than One AntigenProceedings of the National Academy of Sciences, 1972
- Specificity and structure of the myeloma protein produced by mouse plasmacytoma MOPC-460Biochemistry, 1971
- THE BINDING OF NICOTINAMIDE-ADENINE DINUCLEOTIDE TO YEAST D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE: TEMPERATURE-JUMP RELAXATION STUDIES ON THE MECHANISM OF AN ALLOSTERIC ENZYMEProceedings of the National Academy of Sciences, 1966
- On the nature of allosteric transitions: A plausible modelJournal of Molecular Biology, 1965