COMPARATIVE STUDIES ON THE EXPRESSION OF FC-RECEPTORS, IA ANTIGENS, AND BETA-2-MICROGLOBULIN FOLLOWING INVITRO HERPES-SIMPLEX VIRUS-INFECTION OF HUMAN LYMPHOID-CELLS

Abstract

Characteristics of the specificity and affinity of surface Fc-receptors induced on Raji (a Burkitt''s lymphoma-derived B [bone marrow-derived] cell line) and Molt-4 (a T [thymus-derived] cell line derived from a leukemic patient) after herpes simplex virus (HSV) infection were studied together with the expression of other surface markers. Inhibition experiments of EA[erythrocyte-antibody]-rosetting suggested that Raji cells injected by HSV had a higher affinity for soluble aggregated Ig[immunoglobulin]G than for IgG serologically adsorbed onto erythrocytes; an opposite pattern was noted with Molt-4, a non-Burkitt''s lymphoma cell line. Using radiometric and microspectrofluorometric techniques, the expression of Fc-receptors, as detected by their capacity to fix aggregated IgG, paralleled the expression of serologically detectable Fc-receptors and Ia determinants. In contrast to .beta.2-microglobulin, Fc-receptor and Ia antigen expression increased following HSV infection. This suggests a specific induction of these markers on human lymphoid cells following HSV infection. This may be the 1st report showing a specific induction of Fc-receptors and Ia antigens by HSV following in vitro infection of human lymphoid cells.