Population Study of Common Glucose-6-Phosphate Dehydrogenase Mutations in Kuwait

Abstract

DNA samples from 206 unrelated Kuwaitis of both sexes, i.e. 200 randomly selected individuals and 6 glucose-6-phosphate-dehydrogenase (G6PD)-deficient probands, have been analyzed by the PCR/RFLP technique for mutations underlying the most common G6PD-deficient variants (Mediterranean and A–). At the first step all samples were studied for the 563C→T and 376A→G mutations, then the samples positive for 376A→G were further analyzed for 202G→A, 680G→T and 968T→C mutations. Three mutations (563C→T, 376A→G and 202G→A) were found to be present in the Kuwaiti population at polymorphic frequencies (0.0503, 0.0215 and 0.0111, respectively). Nineteen out of 20 unrelated Kuwaiti chromosomes with 563C→T had Mediterranean haplotype as judged by 1311C→T polymorphism. The frequency of G6PD-deficient genotypes was 4.5% (5.73% in males and 2.56% in females).