IN02, A Positive Regulator of Lipid Biosynthesis, Is Essential for the Formation of Inducible Membranes in Yeast
Open Access
- 1 January 2002
- journal article
- research article
- Published by American Society for Cell Biology (ASCB) in Molecular Biology of the Cell
- Vol. 13 (1), 40-51
- https://doi.org/10.1091/mbc.01-07-0366
Abstract
Junctional complexes such as tight junctions (TJ) and adherens junctions are required for maintaining cell surface asymmetry and polarized transport in epithelial cells. We have shown that Rab13 is recruited to junctional complexes from a cytosolic pool after cell–cell contact formation. In this study, we investigate the role of Rab13 in modulating TJ structure and functions in epithelial MDCK cells. We generate stable MDCK cell lines expressing inactive (T22N mutant) and constitutively active (Q67L mutant) Rab13 as GFP-Rab13 chimeras. Expression of GFP-Rab13Q67L delayed the formation of electrically tight epithelial monolayers as monitored by transepithelial electrical resistance (TER) and induced the leakage of small nonionic tracers from the apical domain. It also disrupted the TJ fence diffusion barrier. Freeze-fracture EM analysis revealed that tight junctional structures did not form a continuous belt but rather a discontinuous series of stranded clusters. Immunofluorescence studies showed that the expression of Rab13Q67L delayed the localization of the TJ transmembrane protein, claudin1, at the cell surface. In contrast, the inactive Rab13T22N mutant did not disrupt TJ functions, TJ strand architecture nor claudin1 localization. Our data revealed that Rab13 plays an important role in regulating both the structure and function of tight junctions.Keywords
This publication has 68 references indexed in Scilit:
- Expression of the INO2 regulatory gene of Saccharomyces cerevisiae is controlled by positive and negative promoter elements and an upstream open reading frameMolecular Microbiology, 2004
- High density synthetic oligonucleotide arraysNature Genetics, 1999
- INTRACELLULAR SIGNALING FROM THE ENDOPLASMIC RETICULUM TO THE NUCLEUSAnnual Review of Cell and Developmental Biology, 1998
- Identification of Multiple Forms of 180-kDa Ribosome Receptor in Human CellsDNA and Cell Biology, 1998
- Functional characterization of the 180-kD ribosome receptor in vivo.The Journal of cell biology, 1995
- Myc—Max—Mad: a transcription factor network controlling cell cycle progression, differentiation and deathCurrent Opinion in Genetics & Development, 1994
- Transcriptional induction of genes encoding endoplasmic reticulum resident proteins requires a transmembrane protein kinaseCell, 1993
- The HOP1 gene encodes a meiosis-specific component of yeast chromosomesCell, 1990
- Increased amounts of HMG-CoA reductase induce "karmellae": a proliferation of stacked membrane pairs surrounding the yeast nucleus.The Journal of cell biology, 1988
- CARDIOLIPIN CONTENT OF WILD TYPE AND MUTANT YEASTS IN RELATION TO MITOCHONDRIAL FUNCTION AND DEVELOPMENTThe Journal of cell biology, 1971