Opsin for immunological studies
- 1 January 1985
- journal article
- research article
- Published by Taylor & Francis in Current Eye Research
- Vol. 4 (3), 229-232
- https://doi.org/10.3109/02713688509000854
Abstract
Opsin for immunological studies can be prepared free from S-antigen by affinity chromatography on Con A-Sepharose. This preparation,however,contains 2-6% Con A originating from the affinity medium. Con A as impurity disturbes lymphocyte transformation tests carried out with opsin as test antigen. We describe a method for the removal of Con A by immuno-adsorption to Protein A/anti-Con A/IgG in detergent. In addition, we have selected some detergents and detergent concentrations in which opsin can be purified and added to the lymphocyte culture medi urn.This publication has 6 references indexed in Scilit:
- Opsin-induced experimental autoimmune retinitis in ratsCurrent Eye Research, 1984
- Experimental retinal autoimmunity (ERA) in strain 13 guinea pigs: Induction of ERA-retinopathy with rhodopsinClinical Immunology and Immunopathology, 1983
- IMMUNE REACTIVITY TO DIFFERENT RETINAL ANTIGENS IN PATIENTS SUFFERING FROM RETINITIS PIGMENTOSA1980
- Patterns of Experimental Allergic Uveitis Induced by Rhodopsin and Retinal Rod Outer SegmentsOphthalmic Research, 1980
- A rapid determination of sodium dodecyl sulfate with methylene blueAnalytical Biochemistry, 1975