CHEMICAL AND PHYSICAL FACTORS OF FIBERS ON ENZYME LEAKAGE AND MORPHOLOGICAL CHANGES OF GRANULOCYTES ADHERING TO FIBERS

Abstract

We have evaluated the compatibility of various fibers as the leukocytes separators by the quantification of the amounts of enzymes which were leaked from adhering granulocytes to fibers. When granulocytes adhered to synthetic and natural fibers, changes in the shape of adhering cells and leakage of lysosomal enzymes were observed, depending on the chemical and physical structure of fibers. However, morphological changes and enzyme leakage were not always correlative. Physical factors such as the diameter and the shape of fibers were important. These changes increased as the diameter of fibers decreased. They could be suppressed by coating the surface of fiber with albumin.