Extraction of Phosphatase, Urease, Proteases, Organic Carbon, and Nitrogen from Soil

Abstract

Extractions of three soils at different incubation times demonstrate that 0.14M sodium pyrophosphate at pH 7.1 extracts phosphatase, urease, casein, and benzoylarginamide hydrolyzing proteases. Extraction yields of phosphatase and casein‐hydrolyzing proteases are remarkably high. The most efficient length of extraction depends both on the type of soil and on the selected enzyme.Correlation analyses of extracted enzymes and extracted C and organic N content generally show a significant correlation (p = 0.05 and 0.01).