Gene expression changes during mouse skeletal myoblast differentiation revealed by transcriptional profiling
- 14 August 2002
- journal article
- Published by American Physiological Society in Physiological Genomics
- Vol. 10 (2), 103-111
- https://doi.org/10.1152/physiolgenomics.00011.2002
Abstract
Studies described here utilize high-density oligonucleotide arrays to characterize changes in global mRNA expression patterns during proliferation, cell cycle withdrawal, and terminal differentiation in mouse C2C12 myoblasts. Statistical analyses revealed 629 sequences differentially regulated between proliferating and differentiating myoblasts. These genes were clustered using self-organizing maps to identify sets of coregulated genes and were assigned to functional categories that were analyzed for distribution across expression clusters. Clusters were identified with statistically significant enrichment of functional categories including muscle contraction, cell adhesion, extracellular matrix function, cellular metabolism, mitochondrial transport, DNA replication, cell cycle control, mRNA transcription, and unexpectedly, immune regulation. In addition, functional category enrichment data can be used to predict gene function for numerous differentially regulated expressed sequence tags. The results provide new insight into how genes involved in these cellular processes may play a role in skeletal muscle growth and differentiation.Keywords
This publication has 40 references indexed in Scilit:
- Gene Expression Profile of Aging and Its Retardation by Caloric RestrictionScience, 1999
- The Transcriptional Program in the Response of Human Fibroblasts to SerumScience, 1999
- TRANSCRIPTIONAL CONTROL OF MUSCLE DEVELOPMENT BY MYOCYTE ENHANCER FACTOR-2 (MEF2) PROTEINSAnnual Review of Cell and Developmental Biology, 1998
- Identification of Osteoglycin as a Component of the Vascular MatrixArteriosclerosis, Thrombosis, and Vascular Biology, 1997
- M-Cadherin-Mediated Cell Adhesion and Complex Formation with the Catenins in Myogenic Mouse CellsExperimental Cell Research, 1997
- Defining the regulatory networks for muscle developmentCurrent Opinion in Genetics & Development, 1996
- Extracellular matrix is required for skeletal muscle differentiation but not myogenin expressionJournal of Cellular Biochemistry, 1996
- Molecular Cloning of Caveolin-3, a Novel Member of the Caveolin Gene Family Expressed Predominantly in MuscleJournal of Biological Chemistry, 1996
- Adhesion molecules and skeletal myogenesisSeminars in Developmental Biology, 1995
- Cytoplasmic activation of human nuclear genes in stable heterocaryonsCell, 1983