METHOD FOR THE PRODUCTION OF A PURIFIED DRY Q FEVER VACCINE

Abstract

A fluid Q fever vaccine, low in nitrogen content and capable of eliciting high complement-fixation responses in guinea pigs and a satisfactory number of conversions in man, was prepared by ether extraction of infected yolk sacs at an adjusted pH of 5.7. A partially purified and dried Q fever vaccine was prepared from the fluid material, with no apparent loss in guinea pig immunogenicity, by the sedimentation of the rickettsiae with high speed centrifugation and resuspension in buffered saline containing 2 percent glucose and 0.05 percent formalin.