The Subcellular Distribution of Some Monoamines Following their Intraventricular Injection into the Rat

Abstract

The subcellular distribution of radioactively labelled p-tyramine, octopamine, β-phenylethylamine, tryptamine, and N-methyltryptamine, 30 min after their intraventricular injection, was investigated and compared with the distributions of equimolar amounts of the putative transmitters noradrenaline, dopamine, and 5-hydroxytryptamine injected under identical conditions. The amount of each labelled amine, and any formed labelled amine metabolites, in each subcellular fraction was assessed using chromatographic techniques. The effect of the presence of Na⁺ and various buffer solutions on the distribution of radioactivity between particulate and supernatant fractions, particularly with respect to tryptamine, was investigated.